Types of skin grafts to cover chronic wounds: which one should you choose?


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The use of skin grafts is a traditional technique to promote the healing of acute and chronic wounds. There are different types of skin graft.

Depending on the origin:

  • Autograft or autologous graft: skin obtained from the patient’s own donor site.
  • Allograft or heterologous graft: skin obtained from another person
  • Xenograft or heterograft: skin from other species, such as pigs.
  • Synthetic skin substitutes: manufactured products that work as skin equivalents. They may be epidermal (keratinocyte cultures), dermal or dermoepidermal (artificial skin).

Only autografts are considered permanent. The rest, either because of their composition (degradable by the host) or because of their origin (rejected by the host’s immune response), only provide temporary coverage during a few weeks.

In our daily clinical practice, in chronic wound clinics, we normally use autologous grafts which, depending on their thickness, are classified into:

  • Total skin graft: includes epidermis and dermis. The incision is made up to the subcutaneous tissue and a wedge of skin is obtained. The edges of the resulting defect in the donor site are approximated by sutures.
  • Split-thickness skin graft: its thickness ranges from 0.2 to 0.4 mm. The cutting plane is located above the hair follicles which, by remaining in the donor site, allow healing by secondary intention. They are divided into thin and thick split-thickness skin grafts.
  • Epidermal graft: it is formed solely by epidermis. The traditional methods for obtaining epidermal grafts, based on the suction technique, were tedious. Recently, a device has been commercialized, Cellu Tome, that obtains epidermal micrografts applying heat and negative pressure on the skin. Separation occurs at the level of the lamina lucida, at the dermoepidermal junction.1 The exact mechanism of action of epidermal micrografts in the wound bed is unknown. Clinical experience seems to indicate that it depends on the quality of the recipient bed. Therefore, in many cases wound reepithelization occurs primarily from the edges of the wound and not by the attachment of the grafts, which would behave as promoters of healing by releasing growth factors.1


Back to the title of this post, which type of graft should you choose? It depends on the wound. As we have seen in the previous post, in order to consider that a wound is prepared for skin grafting, it must meet certain requirements, such as an adequate granulation tissue. However, it is necessary to take into account other factors that will influence the outcome of the intervention, such as the location of the wound, the extent, the underlying pathology and the needs or comorbidities of the patient.

  • When the vascular supply of the wound is low, the use of thin grafts is recommended. This a typical finding in our wound clinic.
  • The contraction of the wound will be greater if there is less viable dermis, such as in thin grafts, which worsens the aesthetics and functionality of the scar. An interesting alternative, used predominantly in extensive wounds and in areas where maintaining mobility is essential, is the use of synthetic dermal substitutes in combination with autologous thin partial thickness skin grafts. Dermal substitutes are sheets with a layer formed mainly by bovine or porcine collagen, which is placed on the wound bed, and another protective silicone layer. After a few weeks, when a suitable dermis is achieved, the silicone sheet is removed and the skin graft is placed.
  • The device to obtain epidermal micrografts (Cellu Tome), might represent an interesting alternative to thin partial skin grafts. This method is simple, does not require anesthesia and minimizes complications in the donor site.1 It is especially interesting in cases of pyoderma gangrenosum because, being minimally invasive, there will be no phenomenon of pathergy in the donor site.2 If you want to know more about epidermal grafts, read the post “Differences between epidermal and dermo-epidermal punch grafts”


What techniques do we normally use to obtain autologous split-thickness skin grafts?

A. Mesh graft.

It is obtained with a dermatome and must be performed in an operating theatre. The main benefit of using a graft with grids is that you can save tissue from the donor site (it can expand several times its size). The interest of this meshing is based on the principle of continuous growth of the keratinocytes, which end up filling the free spaces. On the other hand, this lack of continuity allows fluid to drain through the holes in the graft, which prevents the formation of hematoma or seroma.

For graft taking, it is essential an intimate contact between wound bed and the graft, especially the first 3-5 days. This is not always easily achieved by pressing with gauze and bandages, so the concomitant use of negative pressure therapy may be helpful.


B. Punch grafts (also called micrografts)

Punch grafting does not require the use of complex instruments and can be performed in the consultation room. This is the technique that we normally use in our wound clinic. 

There are different methods of obtaining micrografts, which normally have a diameter between 4 and 6 mm. A curette, scissors, scalpel or punch can be used.3 The fragments obtained from the donor site, which will usually be the anterior face of the thigh, are placed with tweezers on the wound bed, a few millimetres apart from each other. The donor site heals by secondary intention, with the placement of a hydrocolloid dressing or alginate, depending on the bleeding (read post “Which dressing do I choose to cover the skin graft donor site?”). The recipient site is covered with a non-adherent interface dressing and a secondary dressing, or directly with a sheet of calcium alginate fibres as the primary dressing and gauze as the secondary dressing. Local pressure will be applied and compression bandage or discharge will be applied, depending on the aetiology. The first dressing change of the grafted area will be carried out after 5-7 days.


Punch grafting procedure used in our wound clinic. A. Recalcitrant chronic ulcer,without infection data and with adequate granulation tissue. B. Punch grafts on the wound. C. Donor site, thigh, with point bleeding. D. Donor site at day 15. E. Clinical evolution at week 2. Complete epithelialisation at week 6.

Graft taking will depend on the wound bed characteristics (good quality of granulation tissue and little slough), the bacterial load, the immobilization in the days following the intervention and a conservative attitude during the first dressing changes.

Even if not all micrografts adhere to the wound bed, the effect is beneficial in healing, as there is a decrease in pain and growth factors are released. It is essential the conservative cleansing in the first cures, avoiding any kind of debridement in order not to remove the micrografts and maintain the microenvironment that has been created. A few touches with a gauze soaked in physiological serum will be sufficient.



  1. Kirsner RS, Bernstein B, Bhatia A, Lantis J, Le L, Lincoln K, Liu P, Rodgers L, Shaw M, Young D. Clinical Experience and Best Practices Using Epidermal Skin Grafts on Wounds. Wounds. 2015 Nov;27(11):282-92
  1. Richmond NA, Lamel SA, Braun LR, Vivas AC, Serena T, Kirsner RS. Epidermal grafting using a novel suction blister-harvesting system for the treatment of pyoderma gangrenosum. JAMA Dermatol. 2014;150(9):999-1000.
  2. Nordström A, Hansson C. Punch-grafting to enhance healing and to reduce pain in complicated leg and foot ulcers. Acta Derm Venereol. 2008;88(4):389-91.






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